PHYTOCHEMICAL CHARACTERISATION AND IN VITRO ANTIOXIDANT POTENTIAL OF CAROM (TRACHYSPERMUM AMMI L.), A TRADITIONAL MEDICINAL PLANT
DOI:
https://doi.org/10.63735/baqa.v26i2.11-27Keywords:
Trachyspermum ammi, Phenolic Content, Flavonoid Content, Anti-Oxidant PotentialAbstract
Background
Trachyspermum ammi L., commonly known as ajwain or carom, is an important medicinal herb in the Apiaceae family, traditionally employed as a stimulant, carminative, and treatment for ailments ranging from asthma and bronchitis to diarrhea and abdominal pain. This study sought to evaluate the diversity of key health-promoting compounds across seventeen different accessions of this plant, moving beyond agronomic traits to a biochemical focus.
Methods
After cultivation, methanolic extracts were prepared from the fresh leaves of each accession to analyze their phytochemical profiles. The research specifically quantified the total phenolic and flavonoid content, two major classes of bioactive compounds renowned for their health benefits and evaluated the corresponding in vitro antioxidant potential using a DPPH free radical scavenging assay.
Results
The results demonstrated significant variation in the levels of these phytochemicals among the different accessions, with many exhibiting substantial concentrations that correlated strongly with potent, dose-dependent antioxidant activity. These findings are crucial as they provide a scientific basis for the traditional use of ajwain, directly linking its therapeutic effects to its high antioxidant content.
Conclusion
The observed biochemical diversity underscores the plant's potential as a rich source of natural antioxidants for nutraceutical or functional food applications. Ultimately, this research highlights the importance of selective cultivation to optimise the medicinal quality of ajwain and deserves further investigation into the specific active constituents responsible for its efficacy.
References
Malhotra SK. Recent advances in seed spices research–a review. Annals of Plant and Soil Res. 2016;18(4):300-8. DOI: https://www.gkvsociety.com/control/uploads/1.pdf.
Green A. Field guide to herbs & spices: how to identify, select, and use virtually every seasoning on the market. Quirk books; 2015. ISBN 1594740828, 9781594740824. Weblink: https://books.google.gm/books?id=87JvDwAAQBAJ&source=gbs_navlinks_s.
Himani Rawat. (Trachyspermum ammi) Ajwain, Health Benefits, Pharmacological Test, Its Uses in Modern Era, and Its Herbal Remedies - A Review. Int J Res Publicat Review 2021;2(10): 663-667. 2021. DOI: https://ijrpr.com/uploads/V2ISSUE10/IJRPR1562.pdf.
Qureshi AA, Kumar KE. Phytochemical constituents and pharmacological activities of Trachyspermum ammi. 2010:955-959. Weblink: https://www.cabidigitallibrary.org/doi/pdf/10.5555/20123401949.
Ishwar Singh IS, Singh VP. Antifungal properties of aqueous and organic solution extracts of seed plants against Aspergillus flavus and A. niger. 2000: 151-157. Weblink: https://www.cabidigitallibrary.org/doi/full/10.5555/20003001692.
Javed I, Iqbal Z, Rahman ZU, Khan FH, Muhammad F, Aslam B, et al. Comparative antihyperlipidaemic efficacy of Trachyspermum ammi extracts in albino rabbits. Pak Veterinary J. 2006;26(1):23. Weblink: https://www.pvj.com.pk/pdf-files/26_1/23-29.pdf.
Faudale M, Viladomat F, Bastida J, Poli F, Codina C. Antioxidant activity and phenolic composition of wild, edible, and medicinal fennel from different Mediterranean countries. J agricultural and food chemistry. 2008;56(6):1912-20. DOI:
Joshi SG. Medicinal plants. Oxford and IBH publishing; 2000. Weblink: .
Hardel DK, Laxmidhar Sahoo LS, Jitendra Patel JP. Pharmacognostic studies on Trachyspermum ammi Linn. a powder analysis. Int J Res Ayurveda & Pharmacy. 2011 ;2(4). Weblink: http://www.ijrap.net/admin/php/uploads/587_pdf.pdf.
Goyal S, Chaturvedi V, Dhingra G, Tanwar S, Sharma K, Singh S. Trachyspermum ammi: a review on traditional and modern pharmacological aspects. Biological Sci. 2022;2(4):324-37. DOI: .
Dhiman P, Soni K, Singh S. Medicinal value of carom seeds–An overview. PharmaTutor. 2014;2(3):119-23. Weblink: https://www.pharmatutor.org/pdf_download/pdf/Vol.%202,%20Issue%203,%20March%202014,%20PharmaTutor,%20Paper-14.pdf .
Dwivedi SN, Mishra RP, Alava S. Phytochemistry, Pharmacological studies and Traditional benefits of Trachyspermum ammi (Linn.) Sprague. Int J pharmacy & life sci. 2012;3(5). Weblink: http://www.ijplsjournal.com/issues%20PDF%20files/may%202012/10.pdf.
Khan N, Jamila N, Ejaz R, Nishan U, Kim KS. Volatile oil, phytochemical, and biological activities evaluation of Trachyspermum ammi seeds by chromatographic and spectroscopic methods. Analytical Letters. 2020;53(6):984-1001. DOI: https://doi.org/10.1080/00032719.2019.1688825.
Hanif MA, Hassan SM, Mughal SS, Rehman A, Hassan SK, Ibrahim A, et al. An overview on ajwain (Trachyspermum ammi) pharmacological effects: current and conventional. Technology. 2021 Apr 30;5(1):1-6. DOI: https://doi.org/10.11648/j.pst.20210501.11.
Anilakumar KR, Saritha V, Khanum F, Bawa AS. Ameliorative effect of ajwain extract on hexachlorocyclohexane-induced lipid peroxidation in rat liver. Food and chemic toxicol. 2009;47(2):279-82. DOI: https://doi.org/10.1016/j.fct.2008.09.061.
Platel K, Srinivasan K. Studies on the influence of dietary spices on food transit time in experimental rats. Nutrition Res. 2001;21(9): 1309-14. DOI: https://doi.org/10.1016/S0271-5317(01)00331-1.
Zaidi SF, Yamada K, Kadowaki M, Usmanghani K, Sugiyama T. Bactericidal activity of medicinal plants, employed for the treatment of gastrointestinal ailments, against Helicobacter pylori. J ethnopharmacol. 2009;121(2):286-91. DOI: https://doi.org/10.1016/j.jep.2008.11.001.
Singh VK, Singh S, Singh S, Singh DK. Effect of active molluscicidal component of spices on different enzyme activities and biogenic amine levels in the nervous tissue of Lymnaeaacuminata. Phytotherapy Research: An Int J Devoted to Pharmacologic and Toxicologic Evaluation of Natural Product Derivatives. 1999; 13(8):649-54. DOI:
https://doi.org/10.1002/(SICI)1099-1573(199912)13:8<649::AID-PTR518>3.0.CO;2-9
Boskabady MH, Shaikhi J. Inhibitory effect of 26 Vol. 26, No.2, July - December, 2025 Baqai J. Health Sci. 27 Vol. 26, No.2, July - December, 2025 Baqai J. Health Sci. Carum copticum on histamine (H1) receptors of isolated guinea-pig tracheal chains. J Ethnopharmacol. 2000;69(3):217-27.DOI: .
Jeet K, Devi N, Narender T, Sunil T, Lalit S, Raneev T. Trachyspermum ammi (ajwain): a comprehensive review. Intres J pharmacy. 2012 ;3(5):133-8. Weblink: https://www.researchgate.net/publication/237168900_Trachyspermum_ammi_A_comprehensive_review.
Falleh H, Ksouri R, Chaieb K, Karray-Bouraoui N, Trabelsi N, Boulaaba M, et al. Phenolic composition of Cynara cardunculus L. organs, and their biological activities. Comptes Rendus. Biologies. 2008;331(5):372-9. DOI:https://doi.org/10.1016/j.crvi.2008.02.008.
Marino M, Bersani C, Comi G. Impedance measurements to study the antimicrobial activity of essential oils from Lamiaceae and Compositae. Int J food microbiol.2001;67(3):187-95. DOI: https://doi.org/10.1016/S0168-1605(01)00447-0.
Ksouri R, Falleh H, Megdiche W, Trabelsi N, Mhamdi B, Chaieb K, et al. Antioxidant and antimicrobial activities of the edible medicinal halophyte Tamarix gallica L. and related polyphenolic constituents. Food and Chemical toxicol. 2009 ;47(8):2083-91. DOI:https://doi.org/10.1016/j.fct.2009.05.040.
Chalchat JC, Özcan MM. Comparative essential oil composition of flowers, leaves and stems of basil (Ocimum basilicum L.) used as herb. Food Chemistry. 2008;110(2):501-3. DOI: .
Iqbal S, Bhanger MI, Anwar F. Antioxidant properties and components of some commercially available varieties of rice bran in Pakistan. Food chemistry. 2005;93(2):265-72. DOI: https://doi.org/10.1016/j.foodchem.2004.09.024.
Motalleb G, Hanachi P, Kua SH, Fauziah O, Asmah R. Evaluation of phenolic content and total antioxidant activity in Berberis vulgaris fruit extract. 2005;3(1): 648-653. DOI: .
Javanmardi J, Stushnoff C, Locke E, Vivanco JM. Antioxidant activity and total phenolic content of Iranian Ocimum accessions. Food chemistry. 2003;83(4):547-50. DOI: https://doi.org/10.1016/S0308-8146(03)00151-1.
Miliauskas G, Venskutonis PR, Van Beek TA. Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food chemistry. 2004;85(2):231-237. DOI: https://doi.org/10.1016/j.foodchem.2003.05.007.
Othman A, Mukhtar NJ, Ismail NS, Chang SK. Phenolics, flavonoids content and antioxidant activities of 4 Malaysian herbal plants. Int Food Res J. 2014;21(2):759-764. Weblink: http://psasir.upm.edu.my/id/eprint/40773/1/51%20IFRJ%2021%20(02)%202014%20Azizah%20360.pdf.
Baba WN, Tabasum Q, Muzzaffar S, Masoodi FA, Wani I, Ganie SA, et al. Some nutraceutical properties of fenugreek seeds and shoots (Trigonella foenum-graecum L.) from the high Himalayan region. Food Biosci.2018;23(1):31- 37. DOI: https://doi.org/10.1016/j.fbio.2018.02.009.
Mariem S, Hanen F, Inès J, Mejdi S, Riadh K. Phenolic profile, biological activities and fraction analysis of the medicinal halophyte Retama raetam. South African J Botany. 2014;94:114-21. DOI: https://doi.org/10.1016/j.sajb.2014.06.010.
Hajlaoui H, Mighri H, Noumi E, Snoussi M, Trabelsi N, Ksouri R, et al. Chemical composition and biological activities of Tunisian Cuminum cyminum L. essential oil: A high effectiveness against Vibrio spp. strains. Food and chemical
Toxicol. 2010 ;48(8-9):2186-2192.
Naili MB, Alghazeer RO, Saleh NA, Al-Najjar AY. Evaluation of antibacterial and antioxidant activities of Artemisia campestris (Astraceae) and Ziziphus lotus (Rhamnacea). Arabian J Chemist. 2010; 3(2):79-84. DOI: https://doi.org/10.1016/j.arabjc.2010.02.002.
Pellegrini N, Colombi B, Salvatore S, Brenna OV, Galaverna G, Del Rio D, et al. Evaluation of antioxidant capacity of some fruit and vegetable foods: efficiency of extraction of a sequence of solvents. J Sci Food and Agriculture. 2007;87(1):103-11. DOI: https://doi.org/10.1002/jsfa.2682.
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