STUDY OF ANTIBIOTIC RESISTANCE PATTERN OF CLINICAL ISOLATES
Abstract
The antibiotic resistance pattern of the forty five clinical isolates (consisting of 33 Gram negative and 12 Gam positive bacteria) were determined, against nine antibiotics including streptomycin, gentamycin, neomycin, tetracycline, kanamycin, ampicillin, amoxicillin, chloramphenicol and vibramycin.
The level of resistance was determined by Replica plate technique and tube dilution method. Minimum Inhibitory Concentration (MIC), MIC50, MIC90 and % of sensitive and resistant organisms were determined. The Gram negative isolates were found resistant to many antibiotics as compared to the Gram positive clinical isolates. Vibramycin was the only antibiotics which was found effective against 85% of the Gram negative and almost all the Gram positive clinical isolates tested. Whereas against the rest of the antibiotics moderate to higher resistance were observed.
The resistance of the clinical isolates was due to the presence of antibiotic resistant plasmid. This was determined by agarose gel electrophoresis. It was found that 75% of the Gram negative and 58% Gram positive clinical isolates have plasmid and probably the higher resistance pattern was due to presence of R-plasmid.
